Year: 2018 | Month: April | Volume 8 | Issue 2
Differentiation of Marek’s Disease Virus Isolates from India by Sequence Analysis of Meq Gene
Abstract:
The main aim of the present study was to investigate sequence diversity and mutations in the Meq gene of Marek’s disease viruses (MDV) isolated in India. To understand the molecular characteristics of MDV Meq gene, the sequence generated from Directorate of Poultry Research (DPR) farm and commercial poultry farms isolates were compared with published sequences of Indian isolates. Sequence analysis showed that all of the isolates contained an open reading frame (ORF) of 1020 nucleotides, which encoded a 339 amino acid peptides. In the present phylogenetic study, Meq gene sequence generated from MDV strains of DPR isolate was found to share a clade with previously published DPR strains depicting homogenecity in MDV strain prevalent in DPR farm of Hyderabad. The Meq gene sequence of MDV strains amplified from commercial poultry farm found to be evolutionary closer with Ludhiana isolates. Amino acid sequence decoded from Meq gene sequence revealed that four consecutive proline repeats are three in number in DPR isolates while it ranges from 4-5 quadruple proline repeats in commercial farms samples. The published MDV strains from India has 4-5 consecutive four proline repeats in strains from the commercial farms. In the present study, a single point mutation was recorded in Meq gene sequence of DPR isolate where proline was replaced by serine at 233 positions. This mutation is recorded for the first time in Meq gene sequence of Indian isolates. The present study shows that the MDV strains from DPR farm were more virulence than other Indian isolates.
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